Separation and quantitation of linoleic acid oxidation products in mammary gland tissue from mice fed low- and high-fat diets

Abstract  We have developed an assay for the isolation and quantitation by gas chromatography-mass spectrometry (GC-MS) of free 9- and 13-hydroxyoctadecadienoic acid (9-HODE, 13-HODE) in the mammary glands of female mice. Internal standa