Cryopreservation of encapsulated gentian axillary buds following 2 step-preculture with sucrose and desiccation

Abstract  Alginate beads containing axillary buds of in vitro-grown gentian (Gentiana scabra Bunge var. buergeri Maxim.), were successfully cryopreserved following 2 step-preculture with sucrose and desiccation. The optimal preculture conditions